ABSTRACT: The streptokinase gene cloned in pTargeT and replicase based pSinCMV vector was transfected in BHK21 cells. The expression was detected using indirect fluorescent antibody, immunoperoxidase and SDS-PAGE/western blot techniques. High level of gene expression was observed in case of both recombinant plasmids. It shows the functionality of both the recombinant plasmids which could be further evaluated in field trials. KEYWORDS: Streptokinase, pSinCMV vector, pTargeT vector, expression, BHK21 cells

ABSTRACT: Ashoka is the most ancient tree of India, generally known as “ashok briksh”, botanist known as Saraca asoca (Roxb.), De.wild. It is multipurpose tree species in agriculture and ethno pharmacology. Saraca asoca play an important role in nitrogen assimilation study. In this study, combination of different concentration of KNO3 substrate (0.025 M, 0.05 M, 0.10 M, 0.15 M, 0.20 M, and 0.25 M) with 0.10M and 0.20M molarities of buffers under different pH i.e. 7.0, 7.1, 7.2, 7.3, 7.4 and 7.5 solutions were tried to determine the optimum substrate and buffer solution for the maximum nitrate reductase activity in the leaves of Saraca asoca. On the basis of results obtained it was inferred that buffer solution of the strength 0.20 M (pH 7.2) and substrate solution 0.25M was observed suitable for the maximum nitrate reductase activity in the leaf blade of Saraca asoca. The aim of this study was to determine high nitrate reductase activity by using different concentration of KNO3 as substrate in the leaves of Saraca asoca at suitable pH. Key words: Saraca asoca, nitrate reductase activity, substrate and buffer solutions.